Z-β-D-HomoAla-OH is primarily utilized in the field of peptide synthesis and medicinal chemistry. It serves as a building block for creating modified peptides, which are often explored for their potential therapeutic properties. The compound is particularly valuable in the development of peptide-based drugs, where its unique structure can enhance stability, bioavailability, or receptor binding affinity. Additionally, it is used in research to study peptide conformation and interactions, aiding in the design of novel bioactive molecules. Its application extends to the development of enzyme inhibitors and probes for biochemical studies, contributing to advancements in drug discovery and molecular biology.