Used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS), where it serves as a building block for constructing peptide chains. The Fmoc group protects the amino group during the synthesis process, while the tBu group protects the hydroxyl group of allo-threonine, preventing unwanted side reactions. This compound is especially valuable in the production of complex peptides and proteins, ensuring high purity and yield. It is commonly employed in pharmaceutical research and development for creating peptide-based drugs, as well as in biochemical studies to investigate protein structure and function. Its stability and compatibility with standard SPPS protocols make it a reliable choice for peptide chemists.