This compound is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amine functionality, and the tert-butyl (tBu) group protects the hydroxyl side chain of the serine residue. This protection strategy ensures selective reactions during peptide chain assembly, preventing unwanted side reactions. It is especially valuable in synthesizing complex peptides for research in biochemistry, drug development, and biotechnology. The compound’s stability and compatibility with standard SPPS protocols make it a reliable choice for producing peptides with high purity and yield.