Used primarily in peptide synthesis, it serves as a protected form of lysine with a biotin tag. The Boc group ensures selective reactions at other sites of the peptide chain, while the biotin moiety allows for affinity purification or detection using streptavidin. This compound is particularly valuable in biochemical research for labeling proteins or peptides, enabling studies on protein-protein interactions, cellular localization, and biotin-streptavidin binding assays. Its applications extend to drug discovery, where it aids in identifying binding partners or tracking molecular pathways. Additionally, it is employed in the development of diagnostic tools and biosensors due to its strong and specific interaction with streptavidin.