This compound is primarily used in peptide synthesis, particularly as a protected form of histidine. The Boc (tert-butyloxycarbonyl) group serves as a protecting group for the amino function, while the Dnp (2,4-dinitrophenyl) group protects the imidazole side chain of histidine. The IPA (isopropyl alcohol) component aids in solubility and handling during synthesis. It is commonly employed in solid-phase peptide synthesis (SPPS) to ensure selective deprotection and coupling reactions, enabling the construction of complex peptides with high precision. Its application is crucial in the development of pharmaceuticals, bioactive peptides, and research involving histidine-containing peptides.