Boc-L-isoleucine hemihydrate is widely used in peptide synthesis as a protecting group for the amino acid isoleucine. The Boc (tert-butyloxycarbonyl) group shields the amino group during the synthesis process, preventing unwanted reactions and ensuring the correct sequence of amino acids in the peptide chain. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps in the stepwise construction of peptides. After the synthesis is complete, the Boc group can be easily removed under acidic conditions, allowing the peptide to fold and function properly. Additionally, it is utilized in the development of pharmaceuticals, including peptide-based drugs, due to its stability and compatibility with various chemical reactions. Its role in producing bioactive peptides makes it essential in research and drug discovery.