This compound is primarily utilized in the field of organic synthesis, particularly in peptide chemistry. It serves as a key building block for the preparation of complex peptides and proteins. The tert-butoxycarbonyl (Boc) group acts as a protective group for amines, ensuring selective reactions during peptide bond formation. Its application is crucial in the development of pharmaceuticals, where precise control over molecular structure is essential. Additionally, it is employed in research settings for studying enzyme mechanisms and protein interactions, aiding in the discovery of new therapeutic agents.