Used in peptide synthesis as a specialized protecting group reagent, particularly in solid-phase peptide synthesis (SPPS). Its structure allows for selective protection of amino groups, enabling stepwise assembly of complex peptides with minimal side reactions. The fluorenyl moiety provides UV detectability, facilitating reaction monitoring during deprotection steps. It is especially useful in synthesizing long or sensitive peptide sequences where orthogonal protection strategies are required. Also employed in the preparation of peptide libraries for drug discovery due to its stability under various coupling conditions and clean cleavage profiles.