Used primarily in peptide synthesis as a protected form of threonine, it ensures specific amino acid incorporation without unwanted side reactions. The tert-butyl group protects the hydroxyl side chain, while the methyl ester protects the carboxyl group, making it a stable intermediate in solid-phase peptide synthesis. It is particularly valuable in the production of complex peptides for pharmaceutical research, enabling the development of therapeutic agents and bioactive compounds. Its application is crucial in maintaining the integrity and functionality of peptides during chemical reactions.