This compound is primarily used in peptide synthesis as a protected form of proline. It serves as a building block for creating more complex peptide structures, particularly in research and pharmaceutical development. The methoxy group (OMe) acts as a protecting group for the carboxyl function, while the hydrochloride (HCl) salt form enhances its stability and solubility in various reaction conditions. It is often employed in solid-phase peptide synthesis (SPPS) to ensure efficient coupling and deprotection steps. Additionally, it finds application in the study of peptide conformation and biological activity, aiding in the development of therapeutic peptides and peptidomimetics.