Z-D-CIS-HYP-OH is primarily utilized in peptide synthesis and biochemical research. It serves as a building block for constructing specific peptide sequences, particularly in studies focusing on the structure and function of peptides. Its cis-configuration is valuable in mimicking natural peptide conformations, aiding in the development of peptide-based drugs and therapeutic agents. Additionally, it is employed in the study of enzyme-substrate interactions and the design of enzyme inhibitors, contributing to advancements in medicinal chemistry and drug discovery.