H-Tyr(Tbu)-Ome Hydrochloride is primarily used in peptide synthesis as a protected tyrosine derivative. The tert-butyl group protects the hydroxyl side chain of tyrosine, preventing unwanted reactions during the peptide assembly process. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where selective deprotection and coupling steps are crucial for building complex peptide sequences. Its application ensures the integrity and functionality of tyrosine residues in the final peptide product, which is essential for biological activity in therapeutic peptides, research tools, and biochemical studies. Additionally, it aids in the study of protein-protein interactions and enzyme mechanisms by allowing precise incorporation of tyrosine in peptide chains.