Z-D-Ser(tBu)-OH is primarily used in peptide synthesis as a building block for creating custom peptides. It is particularly valuable in the production of peptides with specific stereochemistry, as it introduces the D-serine residue, which is less common in nature. This compound is often employed in pharmaceutical research to develop peptide-based drugs, where precise control over amino acid configuration is crucial. It is also utilized in the study of peptide structure and function, helping researchers understand how different amino acid configurations impact biological activity. Additionally, Z-D-Ser(tBu)-OH is used in the synthesis of peptidomimetics, which are compounds designed to mimic the structure and function of natural peptides, often with enhanced stability or bioavailability. Its tert-butyl protecting group ensures stability during the synthesis process, making it a reliable choice for complex peptide assembly.