Z-Pro-NH2 is primarily used in peptide synthesis as a building block for creating complex peptide structures. It serves as a protective group for the amino terminus, ensuring selective reactions during the synthesis process. This compound is particularly valuable in the production of bioactive peptides and pharmaceuticals, where precise control over peptide sequence and structure is essential. Additionally, it finds application in research laboratories for studying peptide folding, stability, and interactions, contributing to advancements in biochemistry and drug development.