Z-His-OMe is primarily used in peptide synthesis as a protected form of histidine. It serves as a building block in the creation of complex peptides and proteins, ensuring the histidine residue remains stable during the reaction process. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps maintain the integrity of the peptide chain. Additionally, Z-His-OMe is utilized in biochemical research to study enzyme mechanisms and protein interactions, as histidine often plays a critical role in catalytic sites. Its application extends to the development of pharmaceuticals, where it aids in the design of peptide-based drugs targeting various diseases.