N-Carbobenzoxy-DL-Leucine is primarily used in peptide synthesis as a protecting group for amino acids. It helps in preventing unwanted side reactions during the formation of peptide bonds. This compound is particularly valuable in organic chemistry and biochemistry research, where precise control over amino acid reactivity is essential. It is also employed in the preparation of various biologically active peptides and pharmaceutical intermediates. Additionally, it finds application in the study of enzyme mechanisms and protein structure-function relationships, providing insights into molecular interactions and biochemical pathways.