Z-BETA-ALA-OSU is widely used in peptide synthesis as an activating agent for carboxyl groups. It facilitates the coupling of amino acids, making it essential in the production of peptides for research and pharmaceutical development. Its application is particularly valuable in solid-phase peptide synthesis, where it ensures efficient and selective reactions. Additionally, it is utilized in the modification of proteins and other biomolecules, enabling the introduction of specific functional groups. This compound is also employed in the preparation of peptide-based drugs, vaccines, and diagnostic tools, contributing to advancements in medicinal chemistry and biotechnology.