Z-Pro-OH is widely used in peptide synthesis as a protecting group for proline. It helps in the selective protection of the amino group during the formation of peptide bonds, ensuring the desired sequence is achieved without unwanted side reactions. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it aids in the stepwise assembly of peptides. Additionally, it is employed in the preparation of peptide-based pharmaceuticals, where precise control over peptide structure is critical for biological activity. Its stability and compatibility with various coupling reagents make it a reliable choice in both research and industrial applications.