N-Carbobenzyloxy-L-leucine is primarily used in peptide synthesis as a protecting group for the amino group of leucine. Its application is crucial in organic chemistry, particularly in the synthesis of complex peptides and proteins. By protecting the amino group, it prevents unwanted reactions during the peptide bond formation process, ensuring the correct sequence and structure of the peptide chain. This compound is also utilized in the preparation of various biologically active peptides and in the study of enzyme inhibitors. Its role in facilitating selective reactions makes it a valuable tool in the development of pharmaceuticals and biochemical research.