This chemical is primarily used in peptide synthesis, particularly in the production of peptides for research and pharmaceutical purposes. It serves as a protected form of histidine, with an acetyl (Ac) group on the N-terminus and the trityl (Trt) group protecting the imidazole side chain during the synthesis process. This dual protection is crucial to prevent unwanted side reactions, ensure the correct sequence of amino acids in the peptide chain, and allow for selective deprotection. It is commonly employed in solid-phase peptide synthesis (SPPS) to create peptides with high purity and specificity. Additionally, it finds application in the development of peptide-based drugs, vaccines, and biochemical studies where precise peptide sequences are required.