Nα-acetyl-D-arginine dihydrate is primarily used in biochemical research as a modified amino acid to study enzyme kinetics and protein interactions. It serves as a substrate or inhibitor in enzymatic assays, particularly for arginine-specific enzymes, helping to elucidate their mechanisms and functions. This compound is also utilized in peptide synthesis, where it acts as a building block to create modified peptides with enhanced stability or specific biological activities. Additionally, it finds application in cell culture studies to investigate cellular uptake and metabolism of acetylated amino acids, contributing to advancements in metabolic and nutritional research. Its role in modulating nitric oxide pathways is also of interest in studies related to cardiovascular and immune system functions.