N-Acetyl-DL-phenylalanine is primarily used in the pharmaceutical and biochemical research fields. It serves as a key intermediate in the synthesis of various peptides and active pharmaceutical ingredients (APIs). Its acetylated form enhances stability and bioavailability, making it valuable in drug development. Additionally, it is utilized in studies related to enzyme kinetics and protein interactions due to its role as a substrate or inhibitor in biochemical assays. In the food industry, it can be employed as a flavor enhancer or precursor in the production of certain food additives. Its chiral nature also makes it useful in stereochemical studies and the development of chiral catalysts.