N-Acetyl-D-tryptophan is primarily utilized in the field of biochemistry and pharmaceutical research. It serves as a key intermediate in the synthesis of peptides and proteins, aiding in the study of their structure and function. This compound is also employed in the development of therapeutic agents, particularly in the formulation of drugs targeting neurological disorders, due to its role in modulating neurotransmitter activity. Additionally, it is used in nutritional supplements to support mental health and improve sleep quality, leveraging its precursor role in serotonin production. In industrial applications, it is sometimes incorporated into cosmetic formulations for its potential skin-soothing and anti-aging properties.