This compound is widely used in peptide synthesis and biochemical research. It serves as a protected form of lysine, where the Nalpha position is shielded by the Fmoc group, and the Nepsilon position is biotinylated. This dual functionality allows for selective deprotection and labeling, making it valuable in solid-phase peptide synthesis (SPPS) for constructing complex peptides. The biotin tag enables affinity purification or detection using streptavidin-based systems, which is particularly useful in protein-protein interaction studies, immunoassays, and bioconjugation strategies. Additionally, it is employed in the development of biotinylated probes for imaging, diagnostics, and targeted drug delivery systems. Its versatility makes it a key reagent in both academic and industrial research settings.