This chemical is widely used in peptide synthesis as a protecting group for histidine. It helps prevent unwanted side reactions during the formation of peptide bonds, ensuring the integrity of the peptide chain. Specifically, the Boc (tert-butyloxycarbonyl) group protects the amino group, while the Trt (trityl) group shields the imidazole side chain of histidine. This dual protection is crucial in solid-phase peptide synthesis (SPPS), where selective deprotection allows for the stepwise assembly of peptides. Its application is particularly important in the synthesis of complex peptides and proteins used in pharmaceutical research, drug development, and biochemical studies.