His-OMe·2HCl is widely used in peptide synthesis as a protected form of histidine. It serves as a building block for creating complex peptides and proteins, ensuring the histidine residue remains stable during the reaction process. This compound is particularly valuable in pharmaceutical research for developing peptide-based drugs, where precise amino acid incorporation is critical. Additionally, it finds applications in biochemical studies to investigate enzyme mechanisms and protein interactions, as histidine often plays a key role in catalytic sites. Its use in solid-phase peptide synthesis (SPPS) enables efficient and high-yield production of peptides for therapeutic and diagnostic purposes.