This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected form of histidine, where the Fmoc (fluorenylmethyloxycarbonyl) group protects the amino group, and the Clt (chlorotrityl) group protects the side chain imidazole ring. This protection is crucial to prevent unwanted reactions during the peptide assembly process. It allows for the selective deprotection of the amino group, enabling the stepwise addition of amino acids to build peptides with high precision. This compound is especially valuable in the synthesis of complex peptides and proteins, where histidine residues play a critical role in structure and function, such as in enzymes and bioactive peptides. Its use ensures efficient and accurate incorporation of histidine into peptide chains, making it a key reagent in peptide chemistry.