This compound is primarily utilized in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the fluorenylmethoxycarbonyl (Fmoc) group acts as a temporary protecting group for the amino functionality. This allows for the sequential addition of amino acids to build peptides with high precision. The imidazole side chain in the structure is significant for peptides that require histidine residues, which play crucial roles in enzyme active sites and metal binding. Its application is essential in producing peptides for pharmaceutical research, drug development, and biochemical studies, enabling the creation of complex peptide structures with specific biological activities.