This chemical is primarily used in peptide synthesis as a protected amino acid derivative. The fluorenylmethoxycarbonyl (Fmoc) group serves as a protective group for the amine functionality during the synthesis process, allowing for selective deprotection and coupling reactions. The nitro group on the phenyl ring can be exploited for further chemical modifications or as a handle for conjugation in the development of peptide-based drugs or biomaterials. Its application is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps in constructing complex peptides with high precision and efficiency. Additionally, the nitro group may be reduced to an amine, enabling further functionalization or cross-linking in the design of targeted therapeutic agents or biochemical probes.