This chemical is primarily used in peptide synthesis as a building block for creating complex peptide structures. It serves as a protected amino acid derivative, where the fluorenylmethoxycarbonyl (Fmoc) group acts as a temporary protecting group for the amino functionality during the synthesis process. This allows for selective deprotection and coupling reactions, enabling the step-by-step assembly of peptides. Its application is crucial in the development of pharmaceuticals, bioactive peptides, and research tools in biochemistry and molecular biology. The compound's specific structure, including the o-tolyl group, can influence the properties of the resulting peptides, such as stability, binding affinity, or biological activity.