This chemical is primarily used in the field of peptide synthesis, where it serves as a key building block for constructing complex peptide chains. Its structure includes a fluorenylmethoxycarbonyl (Fmoc) protecting group, which is widely employed in solid-phase peptide synthesis (SPPS) to protect the amino group during the assembly process. The presence of the o-tolyl group adds steric and electronic properties that can influence the peptide's conformation and interactions. This compound is particularly valuable in the development of custom peptides for research in biochemistry, pharmaceuticals, and drug discovery. It enables the precise incorporation of specific amino acid residues into peptides, which is essential for studying protein function, designing therapeutic agents, or creating bioactive peptides with targeted applications.