D-Serine methyl ester hydrochloride is primarily utilized in biochemical research and pharmaceutical development. It serves as a key intermediate in the synthesis of more complex molecules, particularly in the production of peptides and other biologically active compounds. Its application is significant in studies involving the modulation of NMDA receptors, which are crucial for brain function and have implications in neurological disorders such as schizophrenia and depression. Additionally, it is used in the development of potential therapeutic agents targeting these receptors, aiding in the exploration of treatments for cognitive and psychiatric conditions.