This compound is primarily utilized in the synthesis of peptides, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the tert-butyl (tBu) group protects the threonine side chain hydroxyl group, and the Dicyclohexylamine (DCHA) acts as a counterion to stabilize the carboxylate group. Its application is crucial in producing peptides with specific sequences, especially in pharmaceutical research and drug development. The protection strategy ensures selective reactions during peptide chain elongation, enabling the synthesis of complex peptides with high precision. Additionally, it is employed in the preparation of peptide-based therapeutics, including hormones, antibiotics, and enzyme inhibitors, where the correct stereochemistry and sequence are essential for biological activity.