BOC-DL-Proline ethyl ester is widely used in peptide synthesis as a protecting group for proline. It helps in stabilizing the amino acid during the formation of peptide bonds, ensuring the integrity of the peptide chain. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it prevents unwanted side reactions and facilitates the stepwise assembly of peptides. Additionally, it is employed in the synthesis of complex organic molecules, including pharmaceuticals and bioactive compounds, where selective protection and deprotection of functional groups are required. Its application extends to research and development in medicinal chemistry, aiding in the creation of novel therapeutic agents.