This compound is primarily used in peptide synthesis as a protecting group for glutamic acid. The Boc (tert-butoxycarbonyl) group protects the amino group, while the 9-fluorenylmethyl ester (Fm) group protects the gamma-carboxyl group of glutamic acid. This dual protection allows selective deprotection during peptide chain assembly, enabling precise control over the synthesis process. It is particularly useful in solid-phase peptide synthesis (SPPS) for constructing complex peptides or proteins. The Fm group can be removed under mild basic conditions, while the Boc group requires acidic conditions, offering flexibility in synthetic strategies. Its application is common in pharmaceuticals, biochemistry, and research for developing peptide-based drugs, enzymes, and other bioactive molecules.