This chemical is primarily used in peptide synthesis as a protective group for amino acids. The Boc (tert-butyloxycarbonyl) group safeguards the amino functionality during the synthesis process, while the 4-nitrophenyl ester acts as an activating group, facilitating the formation of peptide bonds. It is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase methods, enabling the controlled and sequential addition of amino acids to build complex peptides. Its application is crucial in the production of bioactive peptides, pharmaceuticals, and research compounds, ensuring high yield and purity in the final product.