This chemical is widely used in peptide synthesis as a protecting group for glutamic acid. It helps in controlling the reactivity of the carboxyl and amino groups during the formation of peptide bonds. The tert-butyl ester group protects the side chain carboxyl group, while the benzyl ester protects the main chain carboxyl group, allowing selective deprotection and coupling steps. This is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase synthesis, where precise control over the sequence and structure of peptides is essential. It is commonly employed in the production of pharmaceuticals, bioactive peptides, and research applications involving peptide chemistry.