Fmoc-His(Boc)-OH is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected form of histidine, where the Fmoc group protects the amino group and the Boc group protects the imidazole side chain. This protection ensures selective deprotection during the synthesis process, allowing for the controlled assembly of peptides. It is especially valuable in synthesizing peptides containing histidine residues, which are crucial for biological activity in many proteins and peptides. Its application extends to the production of therapeutic peptides, research peptides, and peptide-based drugs, where precise sequence control is essential. Additionally, it is used in the development of peptide libraries for drug discovery and biochemical studies.