This compound is primarily used in peptide synthesis as a protecting group for amino acids. The fluorenylmethoxycarbonyl (Fmoc) group protects the amine functionality during the synthesis process, allowing for selective deprotection and coupling reactions. It is particularly useful in solid-phase peptide synthesis (SPPS), where it enables the stepwise construction of peptides with high precision. The tert-butoxy group provides additional protection for carboxylic acid functionalities, ensuring stability during the synthesis. This chemical is essential in the production of peptides for research, pharmaceuticals, and biotechnology applications, where precise control over peptide sequences is critical.