This chemical is primarily used in peptide synthesis as a protecting group for amino acids. The fluorenylmethoxycarbonyl (Fmoc) group protects the amino functionality during the synthesis process, ensuring selective reactions at other sites. The 2,4-dinitrophenyl (Dnp) group serves as an additional protecting group, particularly for side-chain functionalities, enhancing the specificity of the synthesis. This compound is valuable in solid-phase peptide synthesis, enabling the stepwise construction of peptides with high precision. Its application is critical in producing peptides for research, pharmaceuticals, and biotechnology, where controlled and efficient synthesis is essential.