Z-Ile-OSu is commonly used in peptide synthesis as an activated ester. The Z (benzyloxycarbonyl) group protects the α-amino group of the amino acid isoleucine, while the OSu (N-hydroxysuccinimide) ester activates the carboxyl group, facilitating the formation of peptide bonds. This compound is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase peptide synthesis, where it helps in the selective coupling of amino acids. Its application ensures efficient and controlled reactions, minimizing side products and improving yield. Additionally, Z-Ile-OSu is employed in the preparation of custom peptides for research in biochemistry, pharmaceuticals, and biotechnology, aiding in the study of protein structure and function.