This compound is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. Its Fmoc (9-fluorenylmethoxycarbonyl) protecting group allows for selective deprotection under mild basic conditions, such as with piperidine, making it suitable for stepwise peptide chain assembly. The tert-butyl (tBu) group on the threonine side chain provides additional protection against unwanted reactions during synthesis, ensuring the integrity of the peptide structure. It is especially valuable in the production of complex peptides and proteins for research, pharmaceuticals, and biotechnology applications. Its D-allo-threonine configuration is useful for creating peptides with specific stereochemical properties, which can be critical for biological activity or stability.