Used primarily in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a protected amino acid derivative, enabling the incorporation of phosphorylated tyrosine residues into peptide chains. The Fmoc group protects the amino terminus during synthesis, while the phosphate group is protected by benzyl groups, ensuring stability during the reaction process. This compound is crucial for studying phosphorylation events in proteins, as phosphorylation plays a significant role in cellular signaling and regulation. It is also employed in the development of phosphopeptides for biochemical and pharmaceutical research, aiding in the investigation of enzyme-substrate interactions, kinase activity, and drug discovery targeting phosphorylation pathways.