Carboxypeptidase B is widely used in the biotechnology and pharmaceutical industries for the processing of recombinant proteins. It plays a critical role in the removal of C-terminal lysine and arginine residues from proteins, which is essential for achieving homogeneity in therapeutic proteins such as insulin and monoclonal antibodies. This enzyme is particularly valuable in the production of biosimilars and biologics, ensuring the final product meets stringent quality and efficacy standards. Additionally, it is employed in research settings to study protein structure and function, aiding in the development of new biopharmaceuticals. Its specificity and efficiency make it a key tool in protein engineering and purification processes.