Urokinase, derived from human urine, is a thrombolytic enzyme widely used in the medical field to dissolve blood clots. It is particularly effective in treating conditions such as pulmonary embolism, deep vein thrombosis, acute myocardial infarction, and ischemic stroke. By activating plasminogen to plasmin, it breaks down fibrin clots, restoring blood flow in blocked vessels. It is also utilized in catheter clearance to prevent occlusion in intravenous lines. In research, urokinase is employed to study cell migration, tissue remodeling, and cancer metastasis due to its role in extracellular matrix degradation. Its clinical application requires careful monitoring due to the risk of bleeding complications.