Dispase II is widely used in biological research for its ability to dissociate tissues into single cells without causing significant damage. It is particularly valuable in cell culture applications, where it helps in the gentle separation of cells from tissues, such as in the preparation of primary cell cultures. This enzyme is also employed in the isolation of epidermal cells from skin, making it useful in dermatological studies and skin graft procedures. Additionally, Dispase II is utilized in stem cell research to dissociate embryonic stem cells or organoids, ensuring the integrity and viability of the cells. Its neutral protease activity makes it a preferred choice over other enzymes that might be harsher on cell membranes.