Dispase is widely used in cell culture and tissue engineering for its ability to dissociate tissues into single cells without causing significant damage. It is particularly effective in separating epithelial cells from underlying connective tissue, making it a valuable tool in research involving skin, kidney, and other epithelial tissues. In regenerative medicine, Dispase is employed to isolate cells for transplantation and tissue reconstruction. It is also used in the preparation of cell suspensions for flow cytometry and other analytical techniques. Additionally, Dispase plays a role in wound healing studies, where it helps in understanding cell migration and tissue repair processes. Its gentle enzymatic activity ensures minimal disruption to cell surface proteins, preserving cell viability and functionality for downstream applications.