Clostripain from Clostridium histolyticum is primarily used in biochemical research for its ability to cleave peptide bonds specifically at the carboxyl side of arginine residues. This specificity makes it a valuable tool in proteomics for protein sequencing and peptide mapping. It is also employed in the preparation of protein fragments for structural analysis and in the study of protein-protein interactions. Additionally, clostripain is utilized in the development of biopharmaceuticals, where precise protein modification is required. Its application extends to the field of molecular biology, where it aids in the analysis of protein function and the identification of post-translational modifications.