Trypsinogen is primarily used in biomedical research and clinical diagnostics due to its role as a precursor to the digestive enzyme trypsin. It serves as a key marker for pancreatic function, with elevated levels in blood indicating acute pancreatitis or other pancreatic disorders. In laboratory settings, trypsinogen is studied to understand enzyme activation mechanisms, particularly the conversion to active trypsin, which is crucial for protein digestion and cellular signaling pathways. It is also utilized in cell culture techniques, where controlled trypsin activation aids in tissue dissociation and cell harvesting. Additionally, trypsinogen assays are employed in newborn screening for cystic fibrosis, as reduced levels can signal pancreatic insufficiency associated with the disease.