Ficin is a proteolytic enzyme widely used in biomedical and industrial applications due to its ability to break down proteins. It is commonly employed in tissue dissociation procedures, particularly in cell isolation for research and therapeutic purposes, where it helps separate cells by degrading extracellular matrix proteins. In immunology, ficin is used to cleave antibody molecules, generating fragments such as F(ab')₂ for diagnostic and experimental use. It also finds application in the food industry as a meat tenderizer by breaking down tough protein fibers. Additionally, ficin has been explored for its potential in wound debridement, helping remove dead tissue from burns and ulcers. Its strong proteolytic activity makes it useful in some laboratory protocols involving protein digestion and enzyme-linked assays.